Research interest

Multiple Sclerosis (MS) is a demyelinating inflammatory CNS disease in which longterm-disability is still a major clinical problem and correlates with accumulating neuronal injury. Although the question seems simple, we still don’t know how the neurons are damaged and how the chronicity of the disease is sustained. Though we have already quite powerful tools to suppress acute inflammation in the CNS, there is yet no cure of the disease. A detailed mechanistic understanding of molecular actions requires a thorough understanding of cellular neuroimmune interactions. Multiphoton intravital microscopy is very well suited to analyze these processes in a spatiotemporal context. We have used this technique previously to analyze the neuronal damage mediated by T cells in an in vivo setting. We are interested in the mechanisms guiding immune cells, as we could identify a reticular fiber network, which is used as a “highway” for immune cells in the inflamed CNS. And we want to find out if and how immune cells can persist in special micro environments in the CNS, to find out to what extend they support the ongoing neuronal damage. We could identify Th17 cells directly attacking neurons during acute inflammation, thereby causing neuronal dysfunction and damage in experimental autoimmune encephalomyelitis (EAE), a mouse model of MS within a mouse strain carrying a genetically encoded neuronal FRET reporter for intracellular Calcium flux. In close collaboration with Dr. R. Niesner (Biophysical Analysis lab at the DRFZ Öffnet externen Link im aktuellen Fensterhttp://www.drfz.de/biophysikalische-analytik/) and Prof. A.E. Hauser ( Immundynamics lab at the DRFZ and Charité Öffnet externen Link im aktuellen Fensterhttp://www.drfz.de/immundynamik/) we are working on new methods to improve intravital microscopy and we are developing novel techniques for functional imaging, as e.g. for intravital quantification of neuronal damage.

 

 

Contact

Groupleader
Dr. Helena Radbruch

Phone & Fax:
t: +49 30 450 536 498
f: +49 30 450 536 940
 
E-Mail:
helena.radbruch@charite.de

Postal address:
Department of Neuropathology
Charité - Universitätsmedizin Berlin
Campus Charité Mitte
Charitéplatz 1
Virchowweg 15
D-10117 Berlin